Methylation changes in glutation S-transferase P1(GSTP1) gene promoter in human breast cancer and it’s relation to tumor prognosis.

Glutation S-transferase P1 (GSTP1) gene methylation in promoter CpG islands has been described as a specific biomarker for many types of cancer including breast cancer as a tumor suppressor gene . At presents study we found the GSTP1 gene promoter to be methylated in breast cancer tissues.To study the effect of sequence variation on hypermethylated GSTP1 promoter in cancer tissues and non methylated one in normal tissues, we analyzed the cytosine methylation status as epigenetic changes in 50 tumors from patient’s with breast cancer and 50 normal breast tissues that taken from the same breast tumor’s adjacent region.In order to study the promoter methylation status for GSTP1 gene in breast cancer, 40 CpG sites [nucleotide(nt) 197,190,187,185,183,182,176,162,155,152,148,145,141,132,127,124,112,109,101,99,81,77,74,71,54,53,48,47,43,42,40,38,23,22,15,14,13,11,8,4] were screened. The GSTP1 methylation was detected in breast tumors which was associated with higher tumor grade (p=0.000),tumor stage and tumor metastasis.There was also a significant correlation between tumor stage,grade and metastasis status with sites of methylation