Optimization of experimental parameters for the determination of amoxicillin by sensitive spectrophotometric method using synthesized gold nanoparticles

In this study, a sensitive and simple spectrophotometric method has been developed for determination of amoxicillin (AMX) using gold nanoparticles (AuNPs) synthetized by chemical reduction method. This method is based on addition of AMX to AuNPs and nanoparticles aggregation, consequently absorption band of nanoparticles decreased. The optimization of experimental variables was investigated by examining pH, temperature, buffer type and amount, AMX and AuNPs amounts and process time. In the desired condition, the linear calibration graph in the range of 10-250 μg/l was obtained with proper correlation coefficient (R^2=0.99). Limit of detection (LOD) and relative standard deviation (RSD %) were calculated as 0.12 μg/l and 0.61%, respectively. The proposed method was successfully applied to the AMX measurement in pharmaceutical, real water and biological samples.

Keywords:

Spectrophotometry , Amoxicillin , Gold Nanoparticles