Polymorphism of Flagellin gene (fliC) of Clostridium chauvoei isolates from Iran

Flagellums have been reported to play a critical role in the protective immunity in host. In clostridia genera it is coded by flagellin (fliC) gene. Previous southern blot analysis has suggested that Flic gene may has two copies in genome of C. chauvoei. We studied sequences of two copies (fliA and fliB) of flagellin gene in Iranian isolates. In this study, four specific primers for fliA, fliB copies and two universal primers for flagellin (fliC) were designed. Polymerase chain reactions were performed to amplification of fliA and fliB. Two copies of flagellin gene were sequenced and compared with other sequences in GenBank. In phylogenic tree analysis, fliA and fliB sequences were located in separated (clusters) branches. Nucleotide sequence alignment showed that more divergence in fliBs than fliAs sequences. Six highly conserve regions and thirty-one single nucleotide polymorphisms (include thirty specific nucleotide pattern) were found between fliA and fliB sequences. These findings are the first report of Thirty-one novel Single Nucleotide Polymorphisms in fliC gene of Clostridium chauvoei isolates from Iran.

Keywords:

Clostridium chauvoei , Flagellin , Polymorphism ,