In vitro Evaluation of antibacterial efficacy of aqueous extracts of Iranian Native Plants on the Standard Strains of Pseudomonas aeruginosa

Background and Aim: Pseudomonas aeruginosa is the most common cause of nosocomial infections, that known as one of the most resistant bacteria against antibiotics and show resistance to a lot of common treatment. On the same basis, the aim of the present study is evaluation of the antibacterial activity of aqueous extracts of four kinds of Iranian native plants on the growth of P.aeroginosa and compare them with antibiotics.Materials and Methods: Aqueous extracts of herbs were prepared by the soxhlet extractor and after preparing dilution, antimicrobial effect of them was determined by well diffusion and Broth dilution method on 3 different concentration . Results & Conclusion: In this study, the highest anti-bacterial effect have been relative to Hippophae rhamnoides (17.6 mm). The minimum inhibitory concentration of Zataria multiflora Boiss, Juglans regia L and Origanum vulgare was 25% and was recorded 12.5% for Hippophae rhamnoides. At this research, difined all of the herbal extracts have antibacterial effect on the studied strain and this activity due to the culture, origin, growing season and effective material in plants could be different.

Keywords:

Antibacterial activity , Herbal extract , Pseudomonas aeruginosa , Minimum inhibition concentration