Cloning and expression of truncated Staphylococcal Protein A (SpA) in E. Coli

Staphylococcal protein A (SpA) plays an important role in the Staphylococcus aureus pathogenesis. The ombinant SpA is also widely used in biotechnology to purify polyclonal and monoclonal IgG antibodies. In this study, a truncated form of SpA containing five immunoglobulin-binding domains was cloned and expressed in Escherichia coli. The results revealed that SpA was expressed and secreted into the medium. For confirmation, Western blot assay also was performed using IgG anti goat HRP-conjugated antibodies and the result indicated that the protein was expected one.

Cloning and expression of truncated Staphylococcal Protein A (SpA) in E. Coli Keywords:

E. coli , Expression , Cloning , truncated SpA