Identification of microsatellite markers correlated with grain iron content in Iranian prevalent wheat genotypes
Publish place: 8th national biotechnology congress of I.R. Iran & 4th National Conference on biosecurity
Publish Year: 1392
نوع سند: مقاله کنفرانسی
زبان: English
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NBCI08_0091
تاریخ نمایه سازی: 29 شهریور 1394
Abstract:
Iron is one of the most important nutrients in the human diet which according to the high consumption of staple foods such as wheat, its deficiency in these crops would lead to nutritional disorders and related complications. In the present study, 38 Iranian prevalent wheat genotypes have assessed using 30 pairs of microsatellite markers to identify microsatellite markers associated with wheat grain iron content. Results indicated that, grain iron content Ranged from 34-53mg/Kg and a significant difference was observed between grain iron content of studied genotypes. After analyzing the results of microsatellite amplification, the range of alleles per SSR locus was 2-9 with a mean of 4.5 and the mean of polymorphism information content (PIC) Was 0.55. The stepwise regression analysis has been used for estimation of the relationship between microsatellite markers and grain iron content. The results indicated that the three Xwmc617(4A,4B,4D), Xgwm160 (4A) and Xbarc146 (6D,6B,6A) were significantly correlated with wheat grain iron content.
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Authors
Hedieh Badakhshan
Department of Biotechnology, Faculty of Agriculture, University of Kurdistan, Sanadaj, Iran
Namdar Moradi
Ph.D student of agricultural biotechnology, Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari, Iran, moradinamdar@gmail.com
Hadi mohammadzadeh
Master degree in agricultural biotechnology
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