ADSORPTION OF GLYCOLIPID TO THE SURFACE USING PLASMA TECHNOLOGY; ANTIMICROBIAL EVALUATION

Pseudomonas aeruginosa MA01 isolated from spoiled apple in our previous work and identified as glycolipid producer. This glycolipid proved to have great antimicrobial activity. In the present paper, we tried to examine the possibility of glycolipid adsorption on the polypropylene surface, a very applicable polymer in food and pharmaceutical industries, to evaluate antimicrobial properties of surface. For this purpose, the polymeric film was treated using low pressure cold plasma of air gas. Different plasma exposure times of 1, 4, 8 and 12 min were investigated to evaluate surfacecharacteristics. After activating the surface by plasma, the glycolipid was adsorbed on the surface andthe effect of plasma exposure time was studied. AFM analyses was used to see the changes on thepolymeric surface. Contact angle measurements was done in order to evaluate the hydrophobicity of thesurface. The results of the study showed that the produced glycolipid with regards to the plasma operating condition can be successfully attached to the PP surface, however depending on the plasma operating conditions. Antibacterial performance was investigated against B. Subtilis and B. cereus with agar disk diffusion assay. The results of the study showed significant antibacterial activity of polypropylene film with a plasma exposure time of 8 min.

Keywords:

Plasma , glycolipid , antibacterial surface