Phytosynthesis of silver nanoparticle usingUrticadioicaleaf extract in montmorillonite and its antibacterial behavior

The present study reports the plant mediated synthesis of silver nanoparticles (Ag NPs) and silver nanoparticles deposited on montmorillonite (Ag-MMT) using the plant leaf extract of Urticadioica. AgNO3and extract of Urticadioica were used as asilver precursor and reducing agent, respectively. Nanocompositeswere characterized by UV-Vis spectroscopy,X-ray diffraction (XRD),Fourier transfer infrared (FTIR),and Transmission electron microscopy (TEM). The XRD resultsindicated intercalation of Ag NPs into the clay interlayer space.The presence of MMT in green synthesis of Ag NPs was leaded to formation of small particlessizeand more stability than the produced Ag dispersions.The antibacterial activity of Ag NPs and Ag-MMT were investigated against Gram-positive bacteria likeStaphylococcusaureus and Mirococcusluteus and Gram-negative bacteria such asEscherichia coli, andPseudomonas aeruginosa, by the well diffusion method.

Keywords:

Silver nanoparticles , Green synthsis , Montmorillonite , Urticadioica