Preparation and application of graphene oxide-based sertraline-imprinted polymer on dispersive solid-phase extraction from biological samples

In this study, a graphene oxide-based imprinted polymer with sertraline as a template, methacrylic acid(MAA), γ-methacryloxypropyl trimethoxy silane (γ-MAPs) as a functional monomer, ethylene glycol dimethacrylate(EGDMA) as a cross- linker and 2,2 azobis isobutyronitrile (AIBN) as an initiator have been prepared through the crosslinkreaction of polymerization. The composite of molecularly imprinted polymer (MIPs) was fabricated onto the surface ofSiO2/graphene oxide (GO) after incorporation of vinyl group to lead further polymerization reaction. The synthesized MIP has beencharacterized by fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and x-raydiffraction (XRD) analysis.Dispersive solid phase extraction (DSPE) procedure for the extraction of sertraline from urine and plasma samples usingthe prepared MIPs and non-imprinted (NIPs) was evaluated. The effect of different variables such as the sample pH of,extraction time, salt amount, sorbent amount, type and volume of eluent for desorption process were evaluated.Under optimal conditions linear dynamic range of method was 1-250 μg/L. The amount of relative standard deviation ofproposed procedure was 11.1. Finally this method was applied to the determination of sertraline in plasma and urinesamples and satisfactory relative recoveries were achieved.

Keywords:

Graphene oxide , Sertraline-imprinted polymer , Dispersive solid phase extraction , Urine and plasmasamples