IMPROVED MESSENCHMAL STEM CELL RESPONSES TO NANOSTRUCTURED CP-Ti SUBSTRATES PROCESSED BY SEVERE PLASTIC DEFORMATION

Different types of applications for nanotechnology are beginning to emerge in all areas of medicine.Nanostructured materials have been suggested as the next generation of orthopaedic and dental materials due to theirexcellent bulk and surface properties compared with conventional coarse-grained materials This study investigate thebiocompatibility of nanostructured titanium substrates produced by severe plastic deformation using mouse bonemarrow-derived mesenchymal stem cells (mBMMSCs), compared with those of coarse-grained and untreated Tisubstrates. The morphology of spread cells and cell biological experiments including cell attachment and proliferationare investigated on these different samples. Nanostructured Ti substrates show a significantly higher cell attachmentand proliferation compared with coarse-grained and untreated Ti substrates (p < 0.05). Scanning electron microscopy(SEM) analysis of mBMMSCs grown on samples with different microstructures demonstrates significant differences inmorphology with respect to attachment and growth pattern. These results demonstrate the superior mesenchymal stemcell compatibility of nanostructured Ti substrates over coarse-grained and untreated Ti substrates which could lead toearly implant fixation and improved osseointegration.

Keywords:

Severe plastic deformation , Nanostructured titanium , Cell attachment , Cell morphology