Antibacterial activity of crude ethanolic, methanolic and ethyl acetate of plantago ovata affected by salt stress

As far as, salinity stress is the major factor affecting plant production, in this research, theeffect of different salinity stress levels (0 as a control, 25, 50, 100, 200 and 300 mM NaCl) on some of the biochemical parameters of P. ovata ethanolic extract were evaluated for 30 days. Furthermore, the antibacterial activity of ethanolic, methanolic and ethyl acetate extracts of P. ovata samples were determined in each treatment. The results indicated a significant increasing in proline, total flavonoids and total saponins with the enlargement of NaCl in the medium. Moreover the antibacterial activity of P. ovata extracts was increased with increasing salt level in the medium. The increased antibacterial activity with increasing NaCl treatments, suggest that the secondary metabolites are effective on P.ovata’s resistance to salinity stress. It seems these secondary metabolites increase antibacterial activity of P. ovata and protects it from ion-induced oxidative stress, probably due to a common structural skeleton and the phenyl group of those metabolites. Collectively, our results indicate that P. ovata has physiological traits associated with resistance to salinity through increasing antibacterial activity and accumulation of secondary metabolites which made it as useful plant for growingin saline contaminated sites.

Keywords:

Antibacterial activities , Plantago ovata , Salt stress , Salt tolerance , Secondary metabolites