novel vector for study of hepatitis delta virus replication
Publish place: 04th National Biotechnology Congress of Iran
Publish Year: 1384
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
NBCI04_314
تاریخ نمایه سازی: 30 دی 1386
Abstract:
Hepatitis delta virus (HDV) is a highly pathogenic, hepatotropic agent in humans which requires helper function of hepatitis B Virus (HBV) [Makino1987]. The genome is a small (≅1.7 kb) single- stranded circular RNA molecule. HDV possesses on both the genomic stand (the RNA strand found in virions) and the antigenomic strand (a replicative intermediate), independent ribozymes. The ribozyme is capable of self-cleavage and re-ligation indicating its functional analogy to plant viroids [Taylor1987]. But unlike the viroids, the HDV genome codes for only one protein, the delta antigen (HDAg) [Feng –Ming Lin 2003 ]. Because of the circular character of the HDV genome and consistent with it’s resemblance to plants viroids in their need for tandem repeats to initiate replication the cloned HDV cDNA models employed to study HDV replication are constructed as dimmers or trimers [Modahl L.E 2000]. There is no cell culture suscebtible to HDV in vitro. Therefore, the system most commonly used to study HDV replication is application of HDV constructs [Sambrook J 2000]. In this study, we cloned and manipulated the monomeric full-length HDV cDNA to create a novel dimeric infectious plasmid
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Authors
F Behzadian
Depot. Of Virology, Faculty of medical sciences, Tarbiat modarres univ
F Sabahi
Depot. Of Virology, Faculty of medical sciences, Tarbiat modarres univ
M Karimi
Biotechnology Research center, Pasteur Institute of Iran, Tehran
M Maghsoodi
Neuroscience research center, School of medical science,Shaheed beheshti univ