سیویلیکا را در شبکه های اجتماعی دنبال نمایید.

A novel accurate amplification created restriction site method for determination of the wild type and the precore mutant hepatitis B virus (HBV) variants

Publish Year: 1384
Type: Conference paper
Language: English
View: 2,356

متن کامل این Paper منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل Paper (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دانلود نمایند.

این Paper در بخشهای موضوعی زیر دسته بندی شده است:

Export:

Link to this Paper:

Document National Code:

NBCI04_352

Index date: 20 January 2008

A novel accurate amplification created restriction site method for determination of the wild type and the precore mutant hepatitis B virus (HBV) variants abstract

The most common occurring hepatits B virus (HBV) mutation is the G to A mutation at nucleotide 1896 in the precore region. The aim of this study is to develop a novel accurate amplification created restriction site (ACRS) method for determination of the TGG wild type and the TAG precore mutant HBV variants. Two conserved and consensus specific and diagnostic primers introducing BstX I and Xag I cleavage site were designed in order to determine the G1896 wild type and the A1896 precore mutant HBV variants in all HBV genotype.The results of ACRS method were compared with sequencing data. In the ACRS method, three different informative patterns could be distingished for determination of the wild type, the precore mutant and mixed infection HBV variants. The results of ACRS method on thirty HBV isolates revealed the TAG precore mutant in 50% (15/30), the TGG wild type variant in 30% (9/30) and the mixed infection in 20% (6/30). The sequencing data of these samples were in agreement with the ACRS results. The developed ACRS method is a rapid and cost effective technique for detection of both the TGG wild type and the TAG HBV precore mutant variants. It can be performed for follow up of G1896A precore mutant variant in hepatits B virus infected subjects at routine molecular diagostic laboratories.

A novel accurate amplification created restriction site method for determination of the wild type and the precore mutant hepatitis B virus (HBV) variants Keywords:

A novel accurate amplification created restriction site method for determination of the wild type and the precore mutant hepatitis B virus (HBV) variants authors

Samad Amini Bavil-Olayee

Biotechnology Department, Pasteur Institute of Iran، Tehran

Ramin Sarrami-Forooshani

Biotechnology Department, Pasteur Institute of Iran، Tehran

Ahmad Adeli

Biotechnology Department, Pasteur Institute of Iran، Tehran

Fereidoun Mahboubi

Biotechnology Department, Pasteur Institute of Iran، Tehran