Glutenin subunit composition in bread wheat landraces of Iran by SDS-PAGE technique

To identify the high molecular weight Glutenin subunits of 57 wheat landraces of Northwest of Iran by SDS-PAGE an experiment was conducted at Islamic Azad University, Ardabil Branch in 2013. Considerable diversity with respect to three Glu-A1, Glu-B1 and Glu-D1 loci were observed. In Glu-A1 locus 1, 2* and null alleles were found with the frequency of 10.5, 57.9 and 31.6 percent. In Glu-B1, 7 alleles were detected, in which 7+8 allele was the most frequent allele. In Glu-D1 locus, 2+12 and 5+10 were recognized with the frequency of 68.4 and 31.6 percent. The high quality 5+10 subunit pair was recognized in 9 landraces. Quality score of landraces were ranked from 4 to 10 indicating vast genetic diversity among the landraces. Landraces number 1, 20, 23, 27, 51, 52, 36 and 49 had the best composition of HMW glutenin subunits and consequently highest quality score.

Keywords:

Electrophoresis , Landrace , SDS-PAGE , wheat