سیویلیکا را در شبکه های اجتماعی دنبال نمایید.

In vitro application of Matrigel enhances human blastocyst formation and hatching

Publish Year: 1386
Type: Journal paper
Language: English
View: 518

This Paper With 5 Page And PDF Format Ready To Download

Export:

Link to this Paper:

Document National Code:

JR_IJRM-5-4_004

Index date: 6 September 2016

In vitro application of Matrigel enhances human blastocyst formation and hatching abstract

Background: Matrigel (extracellular matrix) can improve the growth of many cell types in vitro. Objective: The aim of the present study was to determine the effect of Matrigel on thedevelopment of 2-4 cells human embryos in culture. Material and Methods: Surplus 2-4 cells human embryos, resulting from ICSI, weredivided into two groups (control and test). Quality of embryos in both groups was morphologically similar. The test group (n=140) was cultured in Hams’ F10supplemented with 10% human serum albumin and 150 μl liquid Matrigel. The control group (n=140) was cultured in the same medium devoid of Matrigel. Embryos werecultured for an additional 4 days and their morphology was assessed every 24 hours. Both groups were then statistically compared. Results: The percentage of the human embryos that reached the morula stage in thecontrol and test groups were 79.2% and 80%, respectively (p>0.05). However, 36.4% of embryos reached the blastocyst stage in the test group as compared to 5.7% in thecontrol group after 144 hours in culture. This difference was statically significant (p <0.01). In addition, culture of embryos on Matrigel and medium versus medium alonesignificantly improved in vitro hatching (25.7% versus 3.5%; p <0.01). Conclusion: Matrigel at low concentration enhances human blastocyst formation and hatching in vitro.

In vitro application of Matrigel enhances human blastocyst formation and hatching Keywords:

In vitro application of Matrigel enhances human blastocyst formation and hatching authors

Marefat Ghaffari Novin

Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran

Mahnaz Heidari

Nanobiotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran.

Mahdi A Akhondi

Reproductive Biotechnology Research Centre, Avesina Research Institute, ACECR, Tehran, Iran.

Mahmood Jeddi Tehrani

Monoclonal Antibody Research Center, Avesina Research Institute, ACECR, Tehran, Iran.