Determination of effective dosage of FSH and hCG in the maturation of preantral follicles and enclosed oocytes in mice

Publish Year: 1387
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJRM-6-4_006

تاریخ نمایه سازی: 16 شهریور 1395

Abstract:

Background: In vitro maturation (IVM) of oocytes reduces the costs and averts the side-effects of gonadotropin stimulation for in vitro fertilization (IVF). Reliable IVM isan intellectual, scientific and clinical challenge with a number of potential applications. Objective: The effect of hCG was evaluated on the timing and regulation of in vitroovulation for the Syrian mice oocytes in the presence and absence of FSH. Materials and Methods: Preantral follicles, isolated from the ovaries of 6 weeks-oldmice, were cultured in TCM-199 medium. The effect of 10-200 mIU/ml FSH and 1.5IU/ml hCG was seen on the follicle maturation, as well as the changes in ovulation capacity of enclosed oocytes, after the incubation period of 6 days at 37 °C, 92%humidity and 5% CO2 in air. Results: 100 mIU/ml FSH showed increased follicle diameter, survival, germinal vesicle breakdown (GVBD) and oocyte maturation rates (p<0.0001). Significantly higher number of follicles showed cumulus attachment when ovulation started within16-24 hours post hCG (97% and 80% respectively; p<0.0001) as compared to the cultures without hCG or when the ovulation time increased from 24 hours post hCG.Combination of FSH and hCG showed 97% (p<0.0001) ovulation as compared to that seen for FSH-containing medium only (81%) or control (10%). Conclusion: The combined administration of 1.5 IU/ml hCG and 100 mIU/ml FSH induces the in vitro follicle maturation, ovulation capacity and proper timing of mice oocytes.

Authors

Aisha Javed

Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd,Iran.Department of Biochemistry, University of Agriculture, Faisalabad, Pakistan.

Saeed Rezaei-Zarchi

Department of Biology, Payame-Noor University, Yazd, Iran.

Morteza Anvari

Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd,Iran.

Madiha Javeed Ghani

Department of Bioinformatics, Government College University, Faisalabad, Pakistan