Ultrastructural and morphometrical study of preimplantation endometrium in superovulated mice treated with progesterone or Sildenafil
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJRM-11-10_004
تاریخ نمایه سازی: 16 شهریور 1395
Abstract:
Background: Endometrial development has an important role in blastocyst adhesion and implantation. During IVF cycles, endometrial development isenhanced by progesterone. Objective: The aim of this study was to compare ultrastructural and morphometrical characteristics of mice uterine endometrium in natural cycle with those insuperovulated cycles received progesterone or Sildenafil. Materials and Methods: In This study, 60 female bulb/c mice were divided into 4groups: a control and 3 experimental; gonadotropin, gonadotropin+ Sildenafil and gonadotropin+ progesterone. In experimental groups the mice superovulated mated.In the gonadotropin+ progesterone and gonadotropin+ Viagra groups, the micerespectively received 1mg progesterone and 3 mg Sildenafil citrate. Their uterine specimens were prepared for morphometrical and ultrastructural study. Height of theepithelial cells was measured, using motic software. Statistical analysis was performed using ANOVA.Results: Microscopy revealed that in control group the cells had numerous apicalmicrovilli and the height of the cells was 20.52±2.43 μm. In gonadotropin+progesterone group, the granules were found in basal and apical portions and cellular height were 17.91±2.78 μm which were significantly shorter than in the control andgonadotropin groups (p<0.001). In this group, the apical membrane also contained pinopodes. In gonadotropin +Sildenafil group, the granules were found in bothapical and basal portions and the height of the cells were 17.60±2.49 μm which weresignificantly shorter than in the control and gonadotropin groups (p<0.001). In this group, pinopodes appeared slightly extensive than the other groups. Conclusion: It is concluded that superovulatory drugs in mice stimulate endometrial maturation but injection of Sildenafil is nearly more positive.
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Authors
Leila Roshangar
Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Jafar Soleimani-Rad
Drug Applied Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Bahman Rashidi
Department of Anatomical Sciences, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Hossein Mazochian
Medical Education Research Center, Tabriz University of Medical Sciences, Tabriz, Iran