Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJRM-14-3_008
تاریخ نمایه سازی: 16 شهریور 1395
Abstract:
Background: Aging contains morphological and functional deterioration in biological systems. D-galactose (D-gal) generates free radicals and accelerates aging. Portulaca oleracea (Purslane) may have protective effect against oxidative stress.Objective: Purslane ethanolic extract effects were evaluated on antioxidant indicesand sex hormone in D-gal aging female mice. Materials and Methods: 48 female NMRI mice (25-35 gr) were randomly divided into, 6 groups: 1- control (normal saline for 45 days), 2- Purslane (200 mg/kg for last 3 weeks), 3-D-gal (500 mg/kg for 45 days), 4-D-gal+Purslane, 5- Aging, 6-Aging+Purslane. Sex hormones, antioxidants and malondialdehyde (MDA) level of ovary and uterus were measured. Histological assessment was also done.Results: In D-gal treated and aging animals, LH and FSH levels were significantly increased (p<0.001) while estrogen and progesterone levels were significantly reduced (p<0.001) in comparison with control group. MDA contents were significantly increased in ovaries and uterus of D-gal and aging groups (p<0.01). Superoxide dismutase (SOD) (p<0.001) and catalase (p<0.01) activities were significantly decreased in both aging and D-gal treated animals. Ovarian follicles were degenerated and atrophy on uterine wall and endometrial glands was observed in D-gal and aging groups. Alteration in hormone levels, MDA contents and antioxidant activity were significantly reversed by Purslane (p<0.05). Purslane could also improve histological changes such as atrophy of endometrium.Conclusion: These findings indicate that Purslane can attenuate aging alternations induced by D-gal and aging in female reproductive system.
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Authors
Akram Ahangarpour
Health Research Institute, Diabetes Research Center, Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Zohreh Lamoochi
Physiology Research Center. Department of Physiology, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
Hadi Fathi Moghaddam
Physiology Research Center. Department of Physiology, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
Seyed Mohamad Taghi Mansouri
Department of Pharmacology, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran