Recognition an antihelminthic drug teratogenic effects on mouse fetus brain tissue using FTIR-MSP

Introduction: The vibrational spectroscopic techniques are simple, reproducible, and non-destructive on tissues, with the minimal sample requirement and preparation steps. This technique has also been used to measure the characterization and discrimination of disease and tissues from normal ones particularly in cancer detection. Researchers are discovering new areas for FTIR biospectroscopy in medicine. The aim of this study was to measure the possibility of FTIR- MSP application for the recognition and detection of fetus abnormalities after the exposure of pregnant mouse to Levamisole (LEV). LEV is an antihelminthic drug with some applications in immune-deficiency as well as colon cancer therapy.Materials and Method: The pregnant mice were injected with LEV (10 mg/kg) on gestation day 9 and fetus was dissected on pregnancy day 15. The fetus were fixed, dehydrated and embedded in paraffin. Sections of brain (10 μm) were prepared from control and treated groups by Microtome and deparaffinized with xylene. The spectra were taken by FTIR-MSP in the region of 4000-400 cm-1. All spectra were normalized to amide II band (1445cm-1) after baseline correction of the entire spectrum.Results and Discussion: Levamisole has caused many changes in the chemical groups’ composition of mice fetus brain biomolecules. The modifications are very much clear at all important biomolecules of nucleic acids, proteins and fatty acids. FTIR spectroscopy is presenting its potential applicability for the teratogenic studies in this project. FTIR spectroscopy can help understanding the effect of different agents on fetus in a chemically guessable way of the structurally altered groups, providing some indication to understand the mechanism of toxicities on fetus.

Keywords:

FTIR-MSP , Levamisole , Mice fetus brain tissue , Teratogen