Effect of the Medium Composition on Formation ofAlpha Amylase by Bacillus licheniformis GMAF438149
Publish place: سومین کنفرانس بین المللی علوم و مهندسی
Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICESCON03_433
تاریخ نمایه سازی: 16 شهریور 1395
Abstract:
A new medium was developed to induce high level of α- amylase from Bacilluslicheniformis GMAF438149.The strain of Bacillus licheniformis was grown on the presence of different carbon , nitrogen and metal ions. The high level α-amylase has been produced when potato starch and xylose were added to medium. Meat extract ,as an organic nitrogen source, had affected on enzyme yield , while inorganic sources were not found to be effective. Calcium chloride increased activity of the produced enzyme ;however, a slight activity was observed by increase of oxygen concentration. The optimum temperature of Bacillus licheniformis GMAF 438149 for α–amylase production was determined 39°c and the optimum pH was 6.8 . Enzyme was stable at elevated temperature ,and was unstable at varied pH values .Inhibitory effects of Zn and Cu were observed on α-amylase production
Keywords:
Medium Composition -Alpha Amylase -enzyme production
Authors
Fahimeh Mahmoudnia
Department of biotechnology ,Faculty of science , Arak research & science Islamic azad university ,Iran.