Electrospun poly(3-hydroxybutyrate)/ multiwalled carbon nanotubes nanocomposite scaffold for tissue engineering application

A series of poly(hydroxybutyrate) (PHB)/Multiwalled Carbon Nanotubes (MWNTs) nanocomposite scaffolds was prepared by electrospinning for tissue engineering applications. The effect of MWNTs content on the structure and properties of composite scaffold was characterized by scanning electron microscopy (SEM) and universal testing machine (UTM). The attachment ability and viability of osteosarcoma cell lines (MG-63) in the presence of the scaffolds were also investigated. Morphological characterization showed that the addition of different amounts of MWNTs increased the average fiber diameter. The analysis of mechanical properties of the PHB/MWNTs composites revealed great improvement over pure PHB scaffold. The attachment and proliferation of MG-63 Cells were significantly increased in the PHB/MWNTs scaffolds compared with the PHB control. Therefore, the PHB/MWNTs composite scaffolds fabricated by electrospinning may be potentially useful in tissue engineering applications, particularly as scaffold for bone and oral tissue regeneration.

Keywords:

PHB , MWNTs , Electrospinning , nanocomposite , Scaffold , Tissue Regeneration