Isolation and molecular identification of endophytic bacteria from wheat and their antagonistic effects against fungal phytopathogens

Introduction: Endophytic bacteria refers to those bacteria that live inside plant tissues without causing apparent damage. Today, many researchers are willing to isolate and identify endophytic fungi, so these pahotogenic plants are evaluated. Some of them are anti-fungal and anti-bacterial compounds which are capable of producing. Some as fertilizer can help plants to grow and may have benefits for the crop, becasude of anti-bacterial and antifungal effect they have. Most recently they have attracted a lot of attention because they can be used and handled instead of a fertile material and chemical germicide. It is a great help to maintain health and the environment Fusarium moniliforme, Fusarium graminearum, Fusarium oxysporum, Macrophomina phaseolina, Fusarium culmorom (LM 2091). Materials and Methods: Accordingly, in the present study Endophytic bacteria are isolated from the roots and stem of wheat. They were cultured on NA culture medium and then samples of the antagonistic effect against 5 pathogenic fungal ability to produce: Fusarium moniliforme, Fusarium graminearum, Fusarium oxysporum, Macrophomina phaseolina, Fusarium culmorom (LM 2091) and 3 fungal biocontrols are: Tricoderma virida (222-35), Tricoderma harizanum (115), Tricoderma asperellum (Fyt 222.2) were studied in vitro using Dual culture. Results: A total of 69 bacteria were isolated from the root and stem of wheat that of all the isolates showed that only 5 bacteria antagonistic effect after sequencing srRNA 16 as Bacillus subtilis strain khazar, Bacillus subtilis strain IRI, M.CH.V Bacillus subtilis strain, Lysinibacillus macroides strain Tethys was registered in NCBI. Conclusion: The present study showed that wheat is Endophyticy contains bacteria that have an inhibitory effect against plant pathogens.

Keywords:

Endophytic , Wheat , Antagonists , Biocontrol , sr RNA 16