PTPN221858C>T gene polymorphisms in Alopecia Areata: A case–control association study in Iranian population

Introduction: Alopecia Areata (AA) is an autoimmune disease characterized by well-circumscribed patches of hair loss especially from the scalp. The gene encoding the protein tyrosine phosphatase, non-receptor type 22 (PTPN22), which is exclusively expressed in immune cells, has been considered as a risk factor associated with a number of autoimmune diseases. The current study has been performed to investigate whether the PTPN221858C>T SNPs predispose to Alopecia Areata in Iranian patients. Materials and methods: The study group comprised 30 patients (13 female and 17 male with mean age 26.3 ±12.5 years) with Alopecia Areata and 15 healthy controls (5 Female and 10 Male with mean age 30.1 ±5.8 years). Genomic DNA was extracted from whole blood using DNG-Plus method. All individuals were genotyped for PTPN22 polymorphisms using the PCR–RFLP analysis. Results: PCR-RFLP results showed that TT was most frequent genotype in both patient and control groups (OR=3.5, 95%CI= 0.3-32.1, p value>0.05). CT genotype was detected in 6.7% and 13.3% of patients and controls, respectively (OR=0.4, 95%CI=0.05-3.67, p value>0.05). Genotype CC was detected in 73.3% of patients and 80% of controls (OR=0.6, 95%CI=0.15-3.08, p value>0.05). Conclusion: The PTPN22 C1858T is not relevant in susceptibility to AA in the Iranian population.

Keywords:

PTPN22 , Polymorphisms , Alopecia Areata , PCR–RFLP