Evaluation of anticancer activity of vanadium (v) shiff base on MKN-45 (human gastric cancer) cell line

Introduction: Gastric cancer (GC) results from the accumulation of numerous genetic and epigenetic alterations in disrupt the cell cycle and the balance between cell proliferation and cell death. Vanadium, is a dietary micronutrient, is yet to be established as an essential part of the human diet. Multiple biochemical and molecular actions of vanadium have been implicated in its inhibitory effects on various tumor cells of human origin. The active nitrogen and oxygen species may induce some damage to the human body. Antioxidants agent such as complex of vanadium are vital substances, which possess the ability to protect the body from damage caused by free radical induced oxidative stress. The objectives of this study were to evaluate Prosopis farcta as a source of natural anticancer agent using hydroalcholic extracting solvent to determine their anticancer capacities of fruit part on MKN-45 (human gastric cancer) cell line. Materials and methods: We have 5000 cells of MKN-45 cell line in 96 cell plate. Then the cells were treated with concentrations of 50, 10 and 1 micrograms of complex schif base ligand vanadium (v). After 24, 48 and 72 hours, the cells were separated by trypsin of the plate. The survival rate and viability of the cells were evaluated by trypan blue staining and MTT test. Results: In this experiment, we found that with increasing concentration and with time, the cell survival reduced, this could be due to activate the apoptosis pathways by Schiff base of vanadium, that can be used potentially as a readily accessible source of natural anticancer, but further work is necessary to determine more clarified. Conclusion: Anticancer activities of polyphenols and flavonoids have been suggested to exert beneficial pharmacological effects on some diseases and cancers as well.

Keywords:

GC , Schif base ligand vanadium (v) , Tripanblue , MTT