Allelic polymorphism of K-casein, β-Lactoglobulin and leptin genes and their association with milk production traits in Iranian Holstein cattle
Publish place: Journal of Cell and Molecular Research، Vol: 5، Issue: 2
Publish Year: 1392
نوع سند: مقاله ژورنالی
زبان: Persian
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شناسه ملی سند علمی:
JR_JCMR-5-2_002
تاریخ نمایه سازی: 7 بهمن 1395
Abstract:
The purpose of this study was to investigate the polymorphism of K-casein (K-CN), β-Lactoglobulin (B-LG) and leptin (LP) genes in Iranian Holstein cattle by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. DNA was extracted from blood samples of 139 cows using a modified phenol chloroform method. Association between K-CN, B-LG and LP genes’ polymorphism with milk production traits were investigated using mixed procedure of SAS software. The frequencies of AA, AB and BB genotypes for K-CN (0.72, 0.18 and 0.10), B-LG (0.43, 0.28 and 0.29) and LP (0.24, 0.63 and 0.13) were also calculated. Statistical results revealed a significant association between AA and BB genotypes of the K-CN gene with milk production and milk protein percentage, respectively. Also, BB genotype of the B-LG gene and AA genotype of the LP gene showed a significant association with protein percentage and milk production (P<0.05), respectively. Therefore, it is feasible to improve milk production traits in these herds using the studied genes
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Authors
Yahya Mohammadi
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Ali Asghar Aslaminejad
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
MohammadReza Nassiri
Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Ali Esmailizadeh Koshkoieh
Department of Animal Science, Faculty of Agriculture, university of Shahid Bahonar , Kerman, Iran