Box-Behnken Experimental Design for Optimizing HSSPME-GC-MS

The goal of the present study is to find the optimal conditions of temperature and extraction time, VOC and NaCl amount, and desorb time of extractionreaction that takes place in the vial while performing the HS-SPME procedure for the determination by GC/MS of six analytes (benzene, toluene, ethylbenzene, ortho xylene, metha xylene and para xylene) and the corresponding IS (MIBK) by fitting and analyzing a response surface for each one. The papershows Box-behnken experimental design (BBD) as a useful technique to improve and optimize an extraction condition and embody the effect that someexperimental factors exert on several analytical responses. A five-factor, three-level BBD was used to optimizing. Forty six batches were prepared andevaluated for responses as dependent variables .In the optimal experimental conditions (2332 gr NaCl,2362 microliter VOC, 02 ◦C extraction temperature, 11 min extraction time and 62 second desorb time) determined by means of a HS-SPME–GC/MS system is carried out..

Keywords:

Box–Behnken , optimization , HS-SPMEGC/MS , BTEX