Somatic embryogenesis in Hypersicum perforatum by using petiole as explant

Because the various beneficial effects of St. John’s wort plant(Hypersicum perforatum), it is used and consumed worldwide.Achieving homogeneity in this plant is the one of the breeding programobjectives. One of the ways to achieve this goal is producing somaticembryos. In this regards, petiole explant of this plant after disinfectionwere cultured in MS medium supplemented with differentconcentrations of 2,4-D (0, 0.5, 1 and 2mg/l) and Kin (0, 0.5, 1 and 2mg/l). After 4 weeks the produced callus transferred in MS mediumsupplemented with different concentrations of BAP (0, 0.5 and 1 mg/l)and NAA (0, 0.5 and 1 mg/l) to differentiate somatic embryos. Theresults showed that the highest amount of embryonic callus productionwere achieved in MS medium supplemented with 1mg/l 2,4-D and 1.5mg/l Kin. In the somatic embryo production stage, the highest amountof somatic embryo obtained in MS medium supplemented with 1mg/lNAA and 1 mg/l BAP.

Keywords:

Somatic embryogenesis , Embryonic callus , Explant , Hormone