Release of Progesterone from PLGA-based In Situ Forming System

The most important appropriate reproductive biotechnologies accessible for cattle are estrous synchronization and artificial insemination. Estrous control can be obtained throughout the use of controlled drug delivery systems characterized by a daily release of progesterone. In the present study, PLGA-based in situ forming system was prepared by injecting some specific amount of solution of PLGA and N-methyl-2-pyrrolidone (NMP) containing progesterone into a buffer phosphate/ethanol (80: 20%) at 37 ºC. The PLGA matrix was characterized by scanning electron microscopy (SEM). Also, the removal of NMP from PLGA matrix was evaluated by HPLC technique. The results show that a slow NMP removal from PLGA matrix is observed due to hydrophobicity of PLGA system causing a decline in water penetration