Antibacterial Activity and Cell Viability Optimization of ZnO/Alg/Chit Nanocomposite films for Wound Dressing Applications

This paper considers influence of ZnO nanoparticles with different concentrations incorporated in Alg/Chit films on antibacterial activities and also toxic effects on fibroblast cells. The films were characterized by EDAX-maps, FTIR, UV-Vis, AAS and biological assays of MTT, AO/PI and disc diffusion method. Antibacterial efficiency enhanced by increasing ZnO concentrations while it showed toxic effects on fibroblast cells at concentration of 15 mM. The cell viability results were also confirmed by AO/PI and cristal violet staining methods. Moreover, according to antibacterial study, E. Coli exhibited more resistance to ZnO than S. Aureus bacteria