Detection of Infectious Laryngotrachities Virus PCR and Nested PCR

n this study a polymerase chain reaction (PCR) was optimized for rapid and sensitive detection of infectious Laryngotrachietis (ILTV). A 443bp DNA fragment of ILTV was amplified. After DNA extraction, PCR was carried out using specific primers and then specificity of the results was confirmed by sequencing of PCR products. Sensitivity of the test was found to be 3 210 EID50 by this method. After using Nested-PCR a 201bp fragment amplified which increased the sensitivity of the test 210 1EID50... using this technique has many advantages compared with other methods that are currently used. Therefore, it can be used as a reliable diagnostic test for rapid detection of ILTV infections.

Keywords:

infectious laryngotrachitis , polymerase chain reaction , Nested-PCR