BOLOGICAL EVALUATION OF ANOVEL NANOCOMPOSTE SCAFFOLD OF HYDROXYAPATTE/LAYERED DOUBLEHYDROXDESIGELATIN FORBONE TISSUE ENGINEERING APPLICATIONS

Bone Tissue Engineering (BTE) composed of four main parts: scaffold, cells, signaling factors and bioreactors. Several materials and composites are suggested as a scaffold for BTE. Bioactivity is one of the most important properties of a BTE scaffold. In this work synthesis of a novel nanocomposite including hydroxyapatite (HA), layered double hydroxides (LDH) and gelatin is carried out and its biological properties were studied. The co-precipitation (pH=11) method was used to prepare the powders in 2 different ratio of HA to LDH, using diammonium hydrogen phosphate, calcium nitrate and aluminum nitrate salts as starting materials. The resulted precipitates were dried. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) analyses were used to characterize the synthesized powders. The results demonstrated the presence of nanocrystals of HA and Ca-LDH. The resulted powders were composed to gelatin via solvent casting method then freez dried. The scaffold was prepared via membrane lamination method from the resulted layers that linked together with gelatin as glue. In order to investigate the scaffold cytotoxicity MTT assay was done on osteosarcoma cell line with the 7 days extract of powders and scaffolds. No toxicresponse was observed in any specimens. The results demonstrated that the HA/LDH/gelatin composite has a good potential for bone tissue engineering applications.

Keywords:

: Hydroxyapatite , Layered double hydroxides , Gelatin , Polymer-ceramic scaffolds , biocompatibility , MTTassayNTRODUCTION