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Protective effects of melatonin on testis histology following acute torsion-detorsion in rats

Publish Year: 1395
Type: Journal paper
Language: English
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JR_IJRM-15-3_003

Index date: 10 September 2017

Protective effects of melatonin on testis histology following acute torsion-detorsion in rats abstract

Background: Testicular damage due to spermatic cord torsion may lead toinfertility. It is probably because of changes in oxidative stress factors such asmalondialdehyde.Objective: To investigate the protective effect of melatonin (MLT), as anantioxidant, on testicular damage induced by acute unilateral spermatic cord torsionand detorsion (T/D) in rats.Materials and Methods: In this experimental study, 48 adult male Wistar rats wererandomly divided into three groups (8 rats/group): sham group underwent rightscrotal surgery only., the T/D group underwent right testicular torsion (for 1 hr) anddetorsion, and the melatonin group underwent right testicular torsion, received 25μg/kg melatonin intraperitoneally immediately after surgery of T/D. Then thehistological parameters and malondialdehyde (MDA) changes were evaluated.Results: Torsion and detorsion decreased the diameter of the tubules significantlycompared to controls (p=0.003). Melatonin could increase the diameter, but it wasnot significant (p=0.26). The heights of the epithelium were constant in sham, T/D,and melatonin groups without any significant difference between groups (p=0.98).Based on Johnsen’s score, spermatogenesis was normal in the sham group. Thetorsion significantly injured all lineage cells (p<0.001). There was no any spermatidor sperm in the seminiferous tubules. Melatonin improved the spermatogenesissignificantly (p=0.02), but could not improve MDA level significantly (p=0.99).Conclusion: Severe degenerative changes of testis were induced by acute unilateralspermatic cord torsion and detorsion in rats, but it had no effect on MDA level.

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Protective effects of melatonin on testis histology following acute torsion-detorsion in rats authors

Mehri Mirhoseini

Ph.D, Amol Faculty of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran

Fereshteh Talebpour Amiri

Ph.D, Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, azandaranUniversity of Medical Sciences, Sari, Iran

Abbas Ali Karimpour Malekshah

Ph.D, Department of Anatomical Sciences, Immunogenetics Research Center, Faculty of Medicine, azandaranUniversity of Medical Sciences, Sari, Iran

Zahra Rezanejad Gatabi

M.Sc, Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran