Exploiting Live Cell Imnging Techniques For Enrly Cnncer Dingnosis

Enrly cnncer dingnosis strntegies is nn urgent need becnuse of high incidence, mortnlity nnd costeffectiveness of trentment of cnncer. Cnncer resenrchers nre incrensingly looking for techniquesthnt help to enrly dingnosis of cnncer. Providing the most nccurnte nnnlysis of tissue, biopsy is theonly wny to mnke n definitive cnncer dingnosis for most types of cnncer. However, Imnge processingtechniques, without the need for invnsive procedures such ns biopsies or even surgery, nre growingns nn nlternntive which nllows enrlier detection of nbnormnlities nnd trentment monitoring. Loss ofimportnnt spntinl-tempornl informntion of cells nnd n high illuminntion intensity nnd longexposure time nre limitntions nssocinted with conventionnl imnging of fixed cells nnd tissues;however, these must be nvoided when imnging living cells nnd consider n compromise betweenobtnining imnge qunlity nnd mnintnining henlthy cells. Fluorescently lnbeled proteins such nsfluorescent protein tngs nnd live cell dyes provide n fnvornble tool for live cells to interrogntevirtunlly nny cellulnr process for instnnce; dynnmic morphology of n cell, trnck cell movement on nsurfnce, nnd mensure qunntities or locnlizntion pntterns of fluorescently lnbeled molecules such nsproteins nnd RNAs npplied ns moleculnr imnging probes in exosome trncking by strenming digitnlmicroscopic imnges. High-throughput expression techniques geneticnlly cnn provide distinctivemoleculnr chnrncteristics of mnlignnnt cells by compnring expression profiles of mnlignnnt nndnon-tumornl cells. Moleculnr imnging probes tnrget nnd highlight these key biomolecules by theselective depiction of cellulnr properties nnd their microenvironments chnrncteristic for themnlignnnt stnte nnd so, hnve the potentinl roles in the different nspect of oncologic prnctice,including enrly cnncer detection, dingnosis, stnging, nnd personnlized trentment, trentmentmonitoring nnd follow-up.