Study of cytotoxicity activity zno nano particles (zno- nps) of brassica napus l pollen on liver cancer ( hepg2)

Cytotoxicity , infection and inflammation are cases effects on splitting of cells, as This show the chance of turning DNA damage to mutations. stem cells have significantly role for living organisms for several reasons. stem cells always divide to restore weaken tissues. Study on stem cells follow to progress knowledge about treating diseases. today cancer have considerable advance in the communities. So the role of stem cells evaluated in the cancer therapy. stem cells can be delivery vector in the cancer therapy. Researcher have exposed the substances at small scale can have important applicationscomparison with the same substances at larger scale. Anticancer drugs should destroy the cancerous cell with the minimum side effect on natural cells. ZnO nanoparticles (ZnO-NPs) are materials withsignificantly property such as targeting treatment. In order to provide an examination to determine amount of cytotoxicity effect on Huvec cell lines and HepG2 cells.The MTT assay is prepared to showthe cell viability of Huvec and HepG2 cells. the both cell lines were treated with concentrations 10,5,2.5,1.25, 0.6 μg/ml for 24 and 48 hours. This viability assay procedures reduction of yellow tetrazolium salt to amount cellular metabolic action as an agent cell viability. Sustainable cells include respiratory mitochondrial dehydrogenases enzymes which decrease the MTT elements to formazan an unsolvable crystalline component similar to profound purple color. Morphological analyses observed by invert microscope. The obtained result indicated Zn O nanoparticles (NPs) have minimal effect on normal cells (Huvec) at the comparison with cancerous cells (HepG2). The suggestion of cytotoxicity activity of ZnO nanoparticles (ZnO-NPs) on HepG2 cells revealed that cancer cells were inhibited with IC50 about 0.7 and 0.6 μg/ml of Zn O nanoparticles(NPs) while the average concentration (IC50) of normal cells is about 4 and 1.2 μg/ml at the same time of treatment. Significant morphological changes were observed in both cell lines, the most important changes including decreased size and cell deformation into spherical.The data showed that the nanoparticle is capable of inhibiting cancer cells at low concentrations, while not having an inhibitory effect on the normal cells at the same concentrations. Perhaps in the future, this nanoparticle can be used to control cancer cells and prevent the progression of cancer in living tissue

Keywords:

Cancer Symptoms , Cancer Prevention , Cell and Cancer , Stem Cells and Cancer , Drugs and Cancer