Investigation of inhibitory effect of synthesized silver nanoparticles by palyganum bistota plant against human breast cancer cells

Nanotechnology is a new approach for countless purposes. This science refers to nanoscale particles and their physical, chemical and biological properties. There are several methods for synthesizing ofnanoparticles that among these, biological methods are more acceptable than other methods because of their biocompatibility and low cost. The use of plants for this method is very important because it canlead to the synthesis of nanoparticles with therapeutic properties. Today, nanoparticles are used expeditiously to prevent and treat of cancer. In some cases, nanoparticles are used to drug delivery ortarget cancer cells for treatment. In fact, most purpose for cancer therapy are focused on using compound with the lowest side effects and maybe some nanoparticles are suitable for this purposebecause of their biological characteristics. This study examines the cytotoxic effects of Ag- nanoparticles on Mcf7 cells. For this purpose the Mcf 7 Cells from the Pasteur Institute Tehran were purchased and cultured in a RPMI 10% (FBS) medium at 37 ° C in 5% Co2 and high humidity. After reaching proper growth (logarithmic phase) the cells were transferred to 96 plates and after 24 hours With three replications exposed to different concentrations of nanoparticles. Then at 24, 48 and finally 72 hours later, the cell viability was examined. For this purpose, the MTT solution was added to eachwell and stored for 4 minutes in darkness, Subsequently MTT was replaced with DMSO and the absorbance was recorded at 570 nm. Morphological alterations of treated cells with nanoparticles compared to untreated cells were studied using inverted microscope. The results show that the silver nanoparticle inhibits cancerous cells in low concentrations with IC50 about 7, 6.5 and 6 μg/ml in 24, 48 and 72 h respectively. The survival rate of the cell 48 hours after treatment decreased from 79% to 2% in 3 and 50 μg/ml respectively. These results confirm the effect of dose on the cytotoxicity of nanoparticles. The morphology of cells showed significant changes in size and shape of cell. The results show that nanoparticles have a significant effect on inhibition of cancer cells, and it may be possible to use this nanoparticle for medical purposes

Keywords:

Breast Cancer , Cancer Prevention , Cell and Cancer , Cancer Treatment and Management , Drugs and Cancer