Invitro cytotoxic activity of silver nanoparticles synthesized by palyganum bistota plant

Cancer is an inappropriate growth of tissue that during it the cells are dividing without any delay and cell death decreases. Today, cancer treatment methods and efforts to discover drugs with minimal sideeffects have been widely considered. Many studies on cancer therapy have shown that silver nanoparticles play an important role in suppressing cancer cells and can induce apoptosis and necrosis in cells. The use of normal cells along with cancer cells is necessary to evaluate the cytotoxic effects of a drug or composition in the living tissue. The use of In-vitro assay is important because of the reduceduse of laboratory animals and the rapid achievement of results. In this survey, the cytotoxic effect of Ag NPs on cancer cells (HT-29) and normal cells (Huvec) 24 h after exposure was assessed. The MTT testis a simple but effective method for investigating the cytotoxic effects of drug on different cells. In this method, the cells are exposed to various concentrations of the treatment after being seeded in 96 wellplates and then, using a MTP solution, according to the protocol, the cell survival rate is evaluated. In this study, both cell lines were exposed to the same concentrations of nanoparticles (0, 3.1, 6.2, 12.5,25μg/ml), and after 24 h the treatment was drained and the MTT solution was added on the cells. After 4 hours incubation, MTT evacuation and DMS were added and eventually absorption wasrecorded at 570 nm. The results of normal cells showed that from 3 to 25 μg/ml concentrations the survival rate decreased from 90% to 47% however, the viability of cells decreased from about 64% toabout 9% in cancerous cells at similar concentrations. Median concentrations (IC50) were reported for normal and cancerous cells 20 and 6μg/ml respectively. The results indicate that the Ag NPs inhibitscancer cells with high potent than normal cells. Because the Ag NPs apply less cytotoxicity to normal cells than cancer cells, the use of these nanoparticles can be proposed in suppressing cancer cells inliving tissue.

Keywords:

Cancer Diagnosis , Cancer Prevention , Cell and Cancer , Cancer Treatment and Management , Drugs and Cancer