Evaluation cytotoxic effect of silver nanoparticles synthesized by amaranthus cruentus on hepatic human hepatocyte carcinoma cancer cells

Cancer a genetical disease resulting from cramping DNA and change the cells situation that couse fastly growth of cells. Although nanotechnology is obvious other sciences.that is very useful to cure thecancer. Today metal nano particles be attended for cure cancer because of other methods problem. Metal nanoparticle produce kind of toxicity human tissues cell culture that increasing oxidative stressand cytokines and after that leads to cell death. The hepatic cancer cells separate from original tumor and release in body. Most of them release to the blood vessels but you can observe the hepatic cancercells in lymph nodes. Sometimes the hepatic cancer cells joins to other tissues and grow and creat tumors that these can injure other tissues. In this discourse we want to examine nanosilver toxicityeffect that produce with green synthesize by using Amaranthus cruentus cancer cells of hepatic (Hep G2). In MTT test release 100 microliter of suspension cells include 50000 cells in the field. after that100 microliter compelet field include containing logarithmic dilutions of the nanoparticle composition at concentrations (0, 62.5, 125,250,500) studied in concentrations field put in 3 separate plate that insideeach of sink of plate there is 96 flat home with equel ratio.then put one of them in 37̊c and 5% co2, 95% humidity around 24h and the second one in same situation around 47h and the last one also in thesame situation in 72h. The end of incubation put them out the sinks of field. After that added to the sinks MTT soluble and put it inside incubation around 4h, after incubation put MTT soluble out fromsinks and added DMSO to sinks. At least we can read light soluble absorbance that produce in 560nm wave-length and we can calculating IC50 cells with standard curved. Percentegi of biodiverfity=ODcontrol /OD semple×100. The cured cells with silver nanoparticle reduce survival cells and IC50 that was obtained for silver nanoparticles incubation times of 24, 48 and 72 hours were about (110/120/130μg/ml ) respectively. The percentage viability of the treated cancer cells, after 24/48/72h, was about 6%, 7.9%, and 5.6% to concentration 500 μg/ml respectively. The silver nanoparticles synthesized byAmaranthus cruentus inhibited the growth of hepatic human cells and induced apoptosis in these cells. Therefore, it may be possible to use this nanoparticle for cancer therapy.

Keywords:

Cancer Prevention , Cell and Cancer , Multidisciplinary Cancer Research , Cancer Treatment and Management , Drugs and Cancer