Effect of cytotoxicity of phytosomal curcumin composition in hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is one of the most common cancer around the world. Supporting the need for identification of novel agents for targeting key signaling pathways to either improve theefficacy of the current therapy or reduce toxicity. There is some evidence that curcumin may have antitumor activity in liver cancer. However, instability at physiological pH, low solubility in water andrapid metabolism results in a low oral bioavailability of curcumin. The aim of current study was to evaluate anti proliferative activity of novel formulated form of curcumin, phytosomal curcumin, in hepatocellular carcinoma Huh-7 cells. The cytotoxic activity of phytosomal curcumin was assessed by MTT assay. The expression pattern of Cyclin D1 was studies by real time RT-PCR method. Statisticalanalysis was performed in Excel software. The results of this study showed that phytosomal curcumin inhibits cell proliferation in a dose dependent manner with IC50 of ~500 μM. Also, the expression ofCyclin D1 was significantly reduced in the phytosomal curcumin-treated group compared to control group. Based on the results of this study, We demonstrated that phytosomal curcumin was able toinhibit the cell proliferation of Huh7 cells through cyclinD1 involved in the Wnt / Akt signaling pathway, which is suggested to be studied in animal models.

Keywords:

Skin Cancer , Cell and Cancer , Nutrition and Cancer , Drugs and Cancer , Chemotherapy