Evaluation of scavenging activity and antioxidant capacity of zno-nps biosynthesized with hyssopus officinalis extract

Nanotechnology is rapidly developing and identifying numerous properties for nanoparticles that these properties depend on the characteristics of the nanoparticle, such as size, volume-to-surface ratio,nanoparticle charge, ect. Extensive surface area and high reagent activity of Zn O nanoparticles separate this nanoparticle from others. The use of plants in the synthesis of nanoparticle is importantbecause of its formation speed and stability. Synthetic nanoparticles from plants, especially medicinal plants, have several biological effects such as antioxidant, antibacterial, antiinflammation and anti-cancer properties. The purpose of this investigation were to evaluate the antioxidant andantiproliferative effects of Zn O nanoparticles synthesis Hyssopus Officinalis Plant. In this study, the amount of inhibition of ABTS and DPPH free radicals by Zn O NPs was investigated. Both of the methods are commonly used and effective methods to determine the antioxidant capacity of the substance. To do this, first, ABTS and DPPH free radicals were prepared and then different concentrations (125, 250, 500 and 1000 μg/ml) of nanoparticles were added to equal volume of free radicals. The absorbance was then measured at 734 and 517 nm respectively. Since ABTS free radicals have a maximum absorption of 734 nm, thus, by inhibiting free radicals and changing the color from green to yellow, the amount of adsorption decreases. The free radicals also have the highest absorbance at 517 nm, and with their inhibition, the color of the solution changed from violet to yellow and the absorption rate reduced. The statistical results showed that the Zn O NPs was able to inhibit ABTS and DPPH radicals with IC50 about 500 and 700 μg/ml. Antioxidant capacity of substance is one of the beneficial therapeutic properties that can be effective in preventing and inhibiting many diseases. As the results showed, the nanoparticles with moderate antioxidant power can be used to prevent certain diseases, including cancer

Keywords:

Cancer Diagnosis , Cancer Prevention , Gene and Cancer , Cell and Cancer , Drugs and Cancer