Investigating the inhibitory effects of cerium oxide nanoparticles on abts free radicals and their toxicity on breast cancer cells

Nanotechnology is included a series of nanoscale techniques which have been used interdisciplinary in different fields such as engineering, biology, medicine, and pharmacology. Cerium oxide nanoparticles pose the biological anti-oxidants activities. The ability of cerium oxide nanoparticles to scavenge reactive oxygen species (ROS) indicated its anticancer potentials. Here, with the help of greenchemistry, in order to reduce hazardous chemical substances, we synthesized cerium oxide nanoparticles using Rubia tinctorum extract, and investigate its antioxidant activities. In addition, its potential in inhibition of cancer cells was evaluated. The MTT assay is cell viability test based on effect of material and exogenous material on alive cells. Here we used breast cancer cell line. Cells were seeded on 96-well plates and incubated for 24 hours. After 24 hours cell were introduced to different concentrations of cerium oxide nanoparticles (0, 1.6, 3.1, 6.2, 12.5, 12.5 and 25 μg/ml). After 24, 48, 72 hours post treatment amount of cell viability was determined using ELIZA reader. Furthermore, ABTS (2,2 -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) chemical is used to measure the antioxidant activity of the substances and its radical form has an absorbance at 734 nm. Reducing with antioxidant resulted in decreasing of the peak absorbance. results showed that cerium oxide nanoparticles synthesized in Rubia tinctorum extract are able to inhibit breast cancer cells and values of IC50 at 24, 48 and 72 hours were 50, 7 and 2.5 μg/ml, respectively.Moreover, results of scavenging ABTS free radicals also shown the antioxidant potentials of cerium oxide nanoparticles, with the IC50 values of 800 μg/ml. findings indicated that cerium oxide nanoparticles synthesized in Rubia tinctorum extract, are able to inhibit free radicals in different concentrations, which increasing of the concentration result in more inhibitory effects. According to the results, the antioxidant and anticancer effects of cerium oxide nanoparticles it was recommended to use these nanoparticles in cancer therapy. However, more studies should be done to confirm its anticancer properties.

Keywords:

Breast Cancer , Cancer Prevention , Cell and Cancer , Targeted Cancer Therapy , Drugs and Cancer