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Identification of Peptides Having Targeting Capability of βb2-Crystallin Fibrils

Publish place: International Conference/Workshop of Inter-Islamic Network on Nanotechnology (INN) on Nanotechnology and Nanomedicine
Publish Year: 1396
Type: Conference paper
Language: English
View: 664

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Link to this Paper:
https://civilica.com/doc/702271

Document National Code:

NTNM01_016

Index date: 26 February 2018

Identification of Peptides Having Targeting Capability of βb2-Crystallin Fibrils abstract

Crystallins are main proteins of the eye lens. Aggregation of lens proteins into insoluble fibrillar structures is believed to be the main reason of cataracts. Today the only effective treatment for cataract is surgery and it is not accessible for all people especially in developing world. It is thought that specific targeting of these fibrillar aggregates could help to development of novel approaches for cataract treatment. βB2-crystallin, one of the main proteins of lens crystallin proteins, was used to form model fibrillar aggregates in vitro. Phage display technique was used to identify peptides that bound to βB2-crystallin fibrils with high affinity. Recombinantly expressed βB2-crystallin was fibrillated in 10% (v/v) trifluoroethanol (TFE) solution. Thioflavin-T (ThT) assay and transmission electron microscopy (TEM) were used to confirm the fibrillation of βB2-crystallin. Specific targeting of identified peptides was analyzed using enzyme-linked immunosorbent assay (ELISA) and competitive inhibition assay.

Identification of Peptides Having Targeting Capability of βb2-Crystallin Fibrils Keywords:

Phage display , βB2-crystallin , fibrillar aggregate , cataract , targeting peptide

Identification of Peptides Having Targeting Capability of βb2-Crystallin Fibrils authors

m Ghaffari Sharaf

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.Ingenuity Lab, University of Alberta, Edmonton, AB, Canada.

s Cetinel

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.Ingenuity Lab, University of Alberta, Edmonton, AB, Canada.

v Semenchenko

National Institute of Nanotechnology, National Research Council, Edmonton, AB, Canada.

l Unsworth

Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, Canada.National Institute of Nanotechnology, National Research Council, Edmonton, AB, Canada.