سیویلیکا را در شبکه های اجتماعی دنبال نمایید.

In silico prediction of substrate specificity of adenylation domains of NRPSs genes of some cyanobacteria strains as a mining tool for natural bioactive compounds

Publish Year: 1396
Type: Conference paper
Language: English
View: 618
این Paper فقط به صورت چکیده توسط دبیرخانه ارسال شده است و فایل کامل قابل دریافت نیست. برای یافتن Papers دارای فایل کامل، از بخش [جستجوی مقالات فارسی] اقدام فرمایید.

نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Export:

Link to this Paper:

Document National Code:

IBIS07_002

Index date: 18 April 2018

In silico prediction of substrate specificity of adenylation domains of NRPSs genes of some cyanobacteria strains as a mining tool for natural bioactive compounds abstract

Cyanobacteria have the ability to produce a wide variety of natural bioactive compounds. Horizontal gene transfer is thought to play a role in the sporadic distribution of bioactive compounds producers among cyanobacteria. The most prevalent cyanobacterial natural products are produced in combinatorial pathways like mixed PKS/NRPS systems. Nonribosomal peptide synthetases (NRPSs), synthesize a diverse array of bioactive small peptides, many of which are used in medicine. Therefore, there is considerable interest in predicting NRPS substrate specificity in order to identification the natural bioactive products. To investigation the relationship between natural product genes and the role of horizontal gene transfer in the evolutionary history, we amplified and sequenced the NRPS genes. Moreover, we used some bioinformatics software tools, in order to prediction of peptide compound and an amino acid activated by a specific unknown NRPS A module by some cyanobacteria strains isolated from different Climatic/Geographic Regions and Habitats. Fischerella sp. Sara 1 KY618863, Nostoc sp. BN KY303912 and three Nostoc sp. isolated from agricultural areas, salt water and dry limestones, respectively, were analysed using a combination of the genome mining softwares, AntiSMASH, and NaPDoS. Moreover, Adenylation domain substrate specificity predictions for NRPS enzymes, the signature sequence and the name of the compound, were made using NRPSpreditor2. The results of bioinformatics analysis showed that, Microcistin synthetase B as natural compound, D A W F L G N V as signature sequences and McyB-M1-Leu as predicted amino acid were detected from Fischerella sp. Sara 1 KY618863, Nostoc sp. BN KY303912. However, NRPS gene fragments could not be amplified from three Nostoc sp. isolated from dry limestones. The negative PCR results from the initial NRPS PCRs were confirmed through subsequent annealing-temperature gradient NRPS PCRs. This result suggests that the ability of lateral gene transfer to produce the natural bioactive compound has been lost during evolutionary history in these strains. The data presented here highlight the need for future studies to define the linkages between Horizontal gene transfer in maintenance of NRPS genes among cyanobacteria distributed in different Climatic/Geographic Regions and Habitats

In silico prediction of substrate specificity of adenylation domains of NRPSs genes of some cyanobacteria strains as a mining tool for natural bioactive compounds Keywords:

In silico prediction of substrate specificity of adenylation domains of NRPSs genes of some cyanobacteria strains as a mining tool for natural bioactive compounds authors

Fatemeh Taghavi Zenuz

Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran

Reza Assareh

Young researched and elites clup Tehran north branch, Islamic Azad University. Tehran, Iran

Ali GhaderiKanzagh

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Bahareh Nowruzi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran