Callus Induction Studies in Different Explants of Podophyllum hexandrum (Royle)
Publish place: Advances in Agricultural Science، Vol: 2، Issue: 4
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_AAAS-2-4_002
تاریخ نمایه سازی: 15 اردیبهشت 1397
Abstract:
A protocol for callus induction and in-vitro regeneration through organogenesis was established for Pophyllum hexandrum Royle (Berberidaceae). Callus was induced on Murashige and Skoog (MS) basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA/IAA) from leaf, stem and rhizome explants of this economically valuable medicinal plants. Our study also persented both the approaches i.e shoot and root multiplication. No significant callusing was observed when MS medium is fortified with 1.0 mg l-l of both 2,4-D and Kn in leaf, stem as well as rhizome explants. However, higher concentration of Kn and 2,4-D (2.5 – 3.0 mgl-l) exhibited best callusing in leaf and better in stem explants. Also, higher concentration of another cytokinin BA (1.5 – 2.0 mgl-l) produced excellent calli when combined with 2.0 mg l-l of NAA in both leaf and stem explants. BA and NAA in the range of 2.0 – 2.5 mg l-l performed better proliferation in callus mass in both explants i.e. stem and leaf respectively whereas lower concentration of BA and NAA (0.5 mgl-l) produced poor calli in these explants. Though rhizome explants showed best callus mass when 1.5 - 2.0 mgl-l of BA was incorporated with 1.5 - 2.0 mg l-l of both the auxins IBA and NAA. Remarkable callus induction was achieved at higher concentration of BA with IAA and NAA. Along with this as the concentration of auxin and cytokinin was increased the induction frequency was also increased.
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Authors
Surjeet Singh Bisht
Department of Basic Science and Humanities, VCSG College of Horticulture, Uttarakhand University of Horticulture & Forestry Bharsar, Pauri ۲۴۶۱۲۳, Uttarakhand, India
Sandeep Bisht
Department of Molecular Biology and Biotechnology, VCSG College of Horticulture, Uttarakhand University of Horticulture & Forestry, Bharsar, Pauri ۲۴۶۱۲۳, Uttarakhand, India