Callus Induction Studies in Different Explants of Podophyllum hexandrum (Royle)

A protocol for callus induction and in-vitro regeneration through organogenesis was established for Pophyllum hexandrum Royle (Berberidaceae). Callus was induced on Murashige and Skoog (MS) basal medium supplemented with cytokinin (Kn or BA) and auxin (2,4-D/IBA/NAA/IAA) from leaf, stem and rhizome explants of this economically valuable medicinal plants. Our study also persented both the approaches i.e shoot and root multiplication. No significant callusing was observed when MS medium is fortified with 1.0 mg l-l of both 2,4-D and Kn in leaf, stem as well as rhizome explants. However, higher concentration of Kn and 2,4-D (2.5 – 3.0 mgl-l) exhibited best callusing in leaf and better in stem explants. Also, higher concentration of another cytokinin BA (1.5 – 2.0 mgl-l) produced excellent calli when combined with 2.0 mg l-l of NAA in both leaf and stem explants. BA and NAA in the range of 2.0 – 2.5 mg l-l performed better proliferation in callus mass in both explants i.e. stem and leaf respectively whereas lower concentration of BA and NAA (0.5 mgl-l) produced poor calli in these explants. Though rhizome explants showed best callus mass when 1.5 - 2.0 mgl-l of BA was incorporated with 1.5 - 2.0 mg l-l of both the auxins IBA and NAA. Remarkable callus induction was achieved at higher concentration of BA with IAA and NAA. Along with this as the concentration of auxin and cytokinin was increased the induction frequency was also increased.

Keywords:

Pophyllum hexandrum , Callus induction , Growth regulator