Development of dispersive liquid-liquid microextraction coupled to UPLC-DAD for detection and determination of Phthalic acid esters in Mouthwash

A dispersive liquid-liquid microextraction (DLLME) coupled with ultra performance liquid chromatographydiode array detector (UPLC-DAD) method for extraction and pre-concentration of four phthalic acid esters (PAEs), consisting diethyl phthalate (DEP), Bis-2-Ethyl Hexyl Phthalate(BEHP), dicyclohexyl phthalate (DCHP), and di-nbutyl phthalate (DnBP) in mouthwash have been described. Excellent linearity (r= 0.9998) was observed over the concentration range of 1-100 ng/mL for the PAEs. The RSD% ranged from 0.3 to 2.4% (n = 5). BEHP was the most common type of PAEs encountered in mouthwash with concentration of more than 99.2 ng. mL-1.

Keywords:

PAEs , DLLME , Mouthwash , UPLC-DAD