Silencing of bach1 gene by small interfering RNA–mediation regulates invasive and expression level of miR-21 and MMP-3 in breast cancer cells

Introduction & Aim: Breast cancer is one of the common cancers especially among women all over the world. Despite clinical and therapeutic developments in recent years, many of patients lose their lives after operation and metastasis. Bach1 gene plays a major role in growth of cancer, invasion, metastasis, and inhibition of apoptosis in tumor cells. The purpose of this study is to knockdown bach1 gene and survey its effect on expression level of miR-21 and MMP-3.Methods: In this experimental study, after designing and making specific siRNA of the bach1 gene, its transfect on MDA-MB-468 cell lines was applied. Expression level of the gene was analyzed by qRT-PCR. Proliferation of the cells were examined by means of trypan blue experiment. Metastasis and cell invasion were studied by examining of MMP-3 expression. At the end, expression level of miR-21 was also examined.Results: A significant decrease in expression of bach1 gene in effective dosage (p=0.001), metastasis and cell invasion was observed and also bach1 knockdown resulted in a significant decrease in expression of mir-21 (p=0.0014) and MMP-3 (p<0.0001).Conclusion: Bach1 knockdown can play a role in decreasing the breast cancer’s impact particularly on metastasis and invasion. In addition, it decreases the expression of metastatic microRNA such as miR-21. Therefore, knockdown of bach1 gene can have an effective therapeutic impact on breast cancer. Bach1 and miR-21 are considered as diagnostic markers in breast cancer.

Keywords:

breast cancer , bach1 , siRNA , miR-21 , MMP-3