Association between mir-125a and mir-30c gene polymorphisms and ischemic stroke; a case control study

Background: Stroke is a complex and multifactorial disorder in which environmental and genetic risk factorsare involved. Several studies have revealed contribution of genetic factors to development of thisphenomenon. This disease is comprised of two main classes: ischemic and hemorrhagic stroke. Ischemicstroke encompasses more than 80% of all cases of stroke.Polymorphisms in miRNA genes can affect theirbiogenesis and function. Contribution of these polymorphisms to many pathologic situations has beenobserved. The present study is to investigate association of mir-30c rs928508 G/A and mir-125a rs12976445C/T with ischemic stroke in population of Northeast Iran (Khorasan Razavi).Method: In this case-control study, 136 ischemic stroke patients were matched for sex and age with 213individuals without any history of vascular diseases, then genotyped for the mentioned polymorphisms byARMS-PCR technique. Statistical analysis on genotyping data was performed by SPSS v.21 software.Results: This study indicates that there is a significant difference in allele distribution between case andcontrol groups. The G allele for mir-30c rs928508 and the C allele for mir-125a rs12976445, may showassociation with ischemic stroke (p-value=0.023; OR=1.42, 95% CI 1.05-1.93 and p-value=0.048; OR=1.37,95% CI 1-1.86 respectively).Conclusion: According to results of the present study, mir-30c rs928508 and mir-125a rs12976445 can beconsidered as susceptibility genetic factors for ischemic stroke in Northeast of Iran.

Keywords:

Ischemic Stroke , miRNA , polymorphism