EVALUATION OF ANTIBACTERIAL EFFECT OF LAVENDER,LAVENDULA ANGUSTIFOLIA,AND MELISSA , MELISSA OFFICIALIS,EXTRACTS ON HUMAN PATHOGENS

Background and Aim:Introduction : Staphylococcus aureus and E.coli are important pathogens in humans. The aim of this study is to investigate the antibacterial effects of Lavender extract and Melissa on gram-negative/positive bacteria of E. coli and Staphylococcus aureus.Methods:Leopheliz plant powder was prepared with three solvents, water, ethanol 80%and methanol80%. To prepare the appropriate concentration of bacteria to determine MIC, we used the MacroFerland half-solution method containing 1.5 * 108CFU / ML for evaluation. The antibacterial activity of these extracts was performed using measuring inhibition zone diameter. Minimum Bactericidal Concentration (MBC) was assessed using disk diffusion method and MIC (Minimum Inhibitory Concentration) with dilution method. The mcg10 gentamicin disk was used as a positive control in this studyResults:The results showed that MBC methanolic extract of Melissa and Lavender with concentration 60 mg / ml were effective on both Staphylococcus aureus and E-coli and MIC with a concentration less than 60 mg / ml extract of lavender methanol and ethanol and ethanol of Melissa had a weak effect on both bacteria and Staphylococcus aureus; respectively, and in the first dilution of microb have not grown.Conclusion:The results showed that the extract of these two plants has mild inhibitory effects on microbial degradation.

Keywords:

MBC.MIC , Lavender , Melissa , E.coli , staphylococcus aureus