MOLECULAR ASSESSMENT OF INTEGRONS IN ISOLATES OF ESCHERICHIA COLI OBTAINED FROM CHILDREN WITH URINARY TRACT INFECTIONS IN KERMANSHAH
Publish place: 19th International Congress of Microbiology of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
MEDISM19_214
تاریخ نمایه سازی: 13 مهر 1397
Abstract:
Background and Aim:The most common manner to spread of antibiotic resistance and appearance of species with multidrug resistance, transmission of resistance genes to antibiotics through integrons. The aim of this study was to determine the prevalence of class I and 2 integrons and its association with drug resistance patterns in isolates of Escherichia coli obtained from children with urinary tract infections in Kermanshah.Methods:The cross-sectional study were collected 89 isolates of Escherichia coli. After determination of isolates by specific biochemical methods, their antibiotic susceptibility was performed by using disk diffusion. The frequency of class I and 2 integrons were determined by using specific primers and PCR methods.Results:Among of 89 isolates 53(59.3%) isolates were multidrug resistance. The highest antibiotic resistance was to Ampicillin (85.4%) and Cotrimoxazole (68.5%) and the most antibiotic sensitivity was to Imipenem (12.4%) and Nitrofurantoin (16.8%). The frequency of class I and 2 integrons were 71.9% and 3.5%, respectively. There was a significant association between the integrons frequency and resistance to tetracycline and gentamicin (P<0.05).Conclusion:This study showed that the high frequency of multidrug resistance isolates, the prevalence of class I and 2 integrons was high in Escherichia coli isolates. As a result, the identification of the integrons frequency and their relationship to drug resistance patterns is important in bacterial isolates.
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Authors
Nahid Madedigoli
Ph.D. Student of Medical Bacteriology, Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Ramin Abiri
Associate Professor, Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran
Amirhooshang Alvandi
Associate Professor, Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran
Jale Moradi
Associate Professor, Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran