PRODIGIOSIN AGAINST SALMONELLA THYPHIMURIUM

Background and Aim:ubject: Prodigiosin is an natural red pigment produced by Serratia marcescens. Prodigiosin has antimicrobial, antimalarial and antitumor properties. Special attention has been paid to effects of silver nanoparticles and it’s the antimicrobial impact due to development of microbial resistance. The aim of this study was comparison of antibacterial effect of prodigiosin, nanosilver and common antibiotic against Salmonella typhimurium.Methods:The standard strains Serratia marcescens PTCC 1111 and Salmonella typhimurium PTCC 1609 were used. Pigment produced by Serratia marcescens was extracted by the acidic methanol. Finally, antimicrobial properties of nanosilver and pigment was investigated by disc diffusion, and microdilution.Results:The inhibition zone of ciprofloxacin, piperacillin, cefpime, ampicemin, gentamicin and azithromycin was 33.0 ± 2.64, 30.66 ± 1.51, 30.33 ± 2.51, 28 ± 2, 19.66 ± 2.51 and 19.66 ± 0.57 mm, respectively. The mean diameter of the inhibition zone of nano silver and prodigiosin was 17.33 ± 0.57 mm and 15.66 ± 0.57 mm, respectively. The MIC and MBC of the nano silver are 6.25 and 12.5 μg/ml. This size is approximately close to the amount of MIC and MBC of some antibiotics such as azithromycin and piperacillin. But prodigiosin has MIC and MBC of 25000 and 50000 μg/ml, respectively.Conclusion:Prodigiosin produced by the Serratia marcescens, has promising bacteriostatic and bactericidal effects and suggests further studies on its use in antibiotic treatment. In this study, nano silver had an appropriate antibacterial activity against Salmonella typhimurium. So, after examining of the antimicrobial properties of nano silver and prodigiosin in In Vivo, they can be used to treat infection with this bacterium.

Keywords:

Prodigiosin , Nano silver , Salmonella typhimurium , Serratia marcescens , Antimicrobial